GB/T 20929-2007 outlines a standardized method for detecting *Acidophilic Thiobacillus ferrooxidans* using gene chip technology. This standard defines key terms, symbols, and abbreviations related to the detection of this bacterium and its activity. It specifies the main technical requirements for sample preparation, including the selection, handling, and processing of test samples. The methodology described in this standard enables efficient and rapid identification of *Thiobacillus ferrooxidans* (Af) through advanced gene chip analysis. Additionally, it provides procedures for assessing the oxidation activity of Af strains within the tested samples, which is crucial for applications in bioleaching and environmental monitoring. This standard serves as a valuable reference for laboratories engaged in microbial detection and functional analysis, ensuring consistency and accuracy in the evaluation of acidophilic bacteria. By integrating molecular biology techniques with high-throughput detection, it enhances the speed and reliability of identifying and characterizing these important microorganisms in various industrial and environmental settings.
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