GB/T 20929-2007 specifies the application of gene chip technology for the detection and activity assessment of *Acidophilic Thiobacillus ferrooxidans*. This standard outlines key terminology, definitions, symbols, and abbreviations related to the detection process. It also details the main technical requirements for sample preparation, including the procedures for creating and analyzing gene chip maps. The standard is designed for the rapid and efficient identification of *Thiobacillus ferrooxidans* (Af) using advanced molecular techniques. In addition to identification, it enables the determination of oxidation activity in Af strains present in the tested samples. This method provides a reliable and high-throughput approach for microbial analysis, particularly useful in environmental and industrial microbiology. The guidelines ensure consistency and accuracy in testing procedures, making it an essential reference for laboratories engaged in the study and monitoring of acidophilic bacteria. By integrating gene chip technology, this standard enhances the speed and precision of microbial detection, supporting applications in bioleaching, bioremediation, and other related fields.
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