**Detection Range:** 20 pg/ml – 480 pg/ml
**Purpose of Use:** This kit is designed to quantitatively determine the level of type I procollagen C-terminal peptide (CICP) in human serum, plasma, and other related biological samples. It is ideal for clinical research and diagnostic applications where accurate measurement of CICP is required.
**Experimental Principle:** The kit employs a double-antibody sandwich ELISA method to detect CICP in the sample. A microplate is pre-coated with a specific monoclonal antibody against human CICP. After adding the sample, the CICP present in the sample binds to the immobilized antibody. An HRP-conjugated secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Following thorough washing, TMB substrate is introduced. Under the catalytic action of HRP, TMB turns blue, and the reaction is stopped by an acidic solution, resulting in a yellow color. The intensity of the color is directly proportional to the concentration of CICP in the sample. The absorbance is measured at 450 nm using a microplate reader, and the concentration of CICP is determined by comparing the sample's OD value to a standard curve.
**Kit Composition:**
1. 30× Washing Solution – 20 ml × 1 bottle
2. Stop Solution – 6 ml × 1 bottle
3. Enzyme Standard Reagent – 6 ml × 1 bottle
4. Enzyme-Labeled Coating Plate – 12 wells × 8 strips
5. Sample Diluent – 6 ml × 1 bottle
6. Color Reagent A – 6 ml × 1 bottle
7. Color Reagent B – 6 ml × 1 bottle
8. Standard Product (960 pg/ml) – 0.5 ml × 1 bottle
9. Standard Diluent – 1.5 ml × 1 bottle
10. Instructions – 1 booklet
11. Sealing Film – 2 sheets
12. Aluminum Bag – 1 bag
This kit is easy to use, highly sensitive, and suitable for high-throughput screening. It ensures reliable results when used according to the provided instructions. Proper storage and handling are essential to maintain the stability and performance of the reagents.
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