This Human Squamous Cell Carcinoma (SCC) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is intended for research use only. It is designed for the quantitative determination of squamous cell carcinoma antigen (SCC) in human serum, plasma, tissue homogenates, cell culture supernatants, and other related biological fluids. The shelf life of the kit is 6 months when stored properly. For optimal performance, store all components at 2–8°C.
The assay is based on the double-antibody sandwich ELISA principle. A microtiter plate is pre-coated with a specific monoclonal antibody against human SCC. After adding the sample, any SCC present in the sample will bind to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then added, forming a complex of antibody-antigen-enzyme-labeled antibody. Unbound materials are washed away, and then a TMB substrate solution is added. Under the catalytic action of HRP, TMB turns blue and then changes to yellow upon addition of an acidic stop solution. The intensity of the color is directly proportional to the concentration of SCC in the sample.
The optical density (OD) is measured at 450 nm using a microplate reader. By comparing the OD values of the samples to a standard curve generated from known concentrations of SCC, the exact concentration of SCC in each sample can be accurately determined. This method ensures high specificity and sensitivity, making it suitable for reliable clinical and research applications. Always follow the manufacturer’s instructions carefully to ensure accurate results.
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