Shanghai Xinzhuang Instrument Co., Ltd. LZ brand solid phase extraction device - solid phase extraction principle Solid Phase Extraction (SPE) is a sample pretreatment technology developed from the mid-1980s. It is developed by a combination of liquid-solid extraction and liquid chromatography. Mainly used for sample separation, purification and enrichment. The main purpose is to reduce sample matrix interference and improve detection sensitivity.
The SPE technology of solid phase extraction device is based on the theory of liquid-solid phase chromatography. The method of selective adsorption and selective elution is used to enrich, separate and purify the sample. It is a physical extraction process including liquid phase and solid phase. It can be approximated as a simple chromatographic process.
The solid phase extraction device SPE is a liquid chromatography separation principle using selective adsorption and selective elution. The more common method is to pass the liquid sample solution through the adsorbent, retain the substance to be tested, and then use the appropriate strength solvent to wash away the impurities, and then quickly elute the test substance with a small amount of solvent, thereby achieving the purpose of rapid separation, purification and concentration. It is also possible to selectively adsorb the interfering impurities and let the measured substance flow out; or simultaneously adsorb the impurities and the tested substance, and then selectively elute the test substance with a suitable solvent.
Solid phase extraction device solid phase extraction operation steps:
The operation is slightly different for the difference in the retention mechanism of the filler (the filler retains the target compound or retains impurities).
Solid phase extraction operations generally have four steps
First, the filler retains the target compound
1. Activation----Remove impurities in the small column and create a certain solvent environment.
2. Load----Dissolve the sample with a certain solvent, transfer it to the column and keep the components on the column.
3, rinse----Maximize the removal of interference.
4. Elution----The substance to be tested is eluted and collected with a small volume of solvent.
Second, the filler retains impurities
Solid phase extraction operations generally have three steps
1. Activation - removes impurities from the column and creates a solvent environment.
2, loading - transfer the sample into the column, at this time most of the target compound will flow out with the sample base, impurities are retained on the column,
So this step is going to start collecting
3. Elution - The components are rinsed down with a small volume of solvent and collected, and the pool is combined.
This is often used to remove pigments in food or pesticide analysis.
For more information about solid phase extraction equipment, please contact Shanghai Xinzhuang Instrument Co., Ltd., Shanghai Xinzhuang Instrument Production and Development of solid phase extraction device 12 holes, 24 holes, unified control, independent control, welcome to inquire!
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