The solid phase extraction device uses a solid adsorbent to adsorb a target compound in a liquid sample, separates it from the matrix of the sample and the interfering compound, and then elutes with an eluent or desorbs by heating to achieve the purpose of separating and enriching the target compound ( That is, separation, purification and enrichment of samples, the purpose is to reduce sample matrix interference and improve detection sensitivity. It is applied to various food safety testing, agricultural product residue monitoring, medical and health, environmental protection, commodity inspection, tap water and chemical production laboratories. .
Here are some common questions about solid phase extraction:
First, which type of loading method is most suitable for solid phase extraction?
At present, positive pressure loading and negative pressure loading are commonly used in the market, but negative pressure loading is caused by inability to effectively control the flow rate, resulting in unstable recovery rate and poor instrument repeatability. Therefore, mainstream manufacturers generally use positive pressure loading. In this way, the automatic solid phase extractor uses a high-precision syringe pump to pressurize the sample to elute the sample, and more precisely control the liquid flow rate, thereby significantly improving the repeatability of the instrument and ensuring the sample recovery rate.
Second, what is the requirement for automatic solid phase extraction sample processing speed?
The solid phase extraction cycle of a single sample is generally about 20 minutes, and the general manufacturer's solid phase extraction apparatus is similar. Therefore, in general, the more samples the instrument processes simultaneously, the faster the processing speed. Fully automated solid phase extraction with 6 channels + 3 modes for flexible extraction, maximizing sample processing speed.
3. Can the automatic solid phase extraction instrument effectively avoid the blockage of the flow path during the extraction process?
Common problems encountered in the application of automatic solid phase extraction instrument: the instrument is faulty due to blockage of the flow path.
In the event of column plugging, most solid phase extractors are not automatically detected, causing samples and solvents to leak, causing severe losses and cross-contamination. The automatic solid phase extraction instrument flow path has a built-in high-sensitivity pressure sensor. When the flow path pressure is >0.6Mpa, the system will automatically alarm, thus effectively protecting the device.
4. Can the automatic solid phase extraction instrument effectively control cross-contamination?
In some automatic solid phase extraction instruments, the sample and solvent use the same pipeline, which is easy to cause pollution. Especially for instruments without cleaning function, a lot of compounds will remain in the pipeline in a short time, which is the root cause of many experimental failures. . The automatic solid phase extraction instrument uses a flow separation unit to automatically select a solvent, and the organic solvent waste liquid has an independent waste liquid passage to effectively avoid cross contamination.
5. How do users choose the right solid phase extraction method?
The easiest way to establish a solid phase extraction method is to look it up from published standards or materials from SPE column manufacturers. However, since the sample matrix to be faced may vary and even the target compound is different, the methods provided in the literature are not necessarily the most optimized method. Therefore, the extraction parameters in these methods need to be optimized.
The establishment of a solid phase extraction method is divided into three steps:
(a) bringing together relevant information;
(b) establishing a preliminary solid phase extraction method;
(c) optimizing the parameters in the method;
Here are some common questions about solid phase extraction:
First, which type of loading method is most suitable for solid phase extraction?
At present, positive pressure loading and negative pressure loading are commonly used in the market, but negative pressure loading is caused by inability to effectively control the flow rate, resulting in unstable recovery rate and poor instrument repeatability. Therefore, mainstream manufacturers generally use positive pressure loading. In this way, the automatic solid phase extractor uses a high-precision syringe pump to pressurize the sample to elute the sample, and more precisely control the liquid flow rate, thereby significantly improving the repeatability of the instrument and ensuring the sample recovery rate.
Second, what is the requirement for automatic solid phase extraction sample processing speed?
The solid phase extraction cycle of a single sample is generally about 20 minutes, and the general manufacturer's solid phase extraction apparatus is similar. Therefore, in general, the more samples the instrument processes simultaneously, the faster the processing speed. Fully automated solid phase extraction with 6 channels + 3 modes for flexible extraction, maximizing sample processing speed.
3. Can the automatic solid phase extraction instrument effectively avoid the blockage of the flow path during the extraction process?
Common problems encountered in the application of automatic solid phase extraction instrument: the instrument is faulty due to blockage of the flow path.
In the event of column plugging, most solid phase extractors are not automatically detected, causing samples and solvents to leak, causing severe losses and cross-contamination. The automatic solid phase extraction instrument flow path has a built-in high-sensitivity pressure sensor. When the flow path pressure is >0.6Mpa, the system will automatically alarm, thus effectively protecting the device.
4. Can the automatic solid phase extraction instrument effectively control cross-contamination?
In some automatic solid phase extraction instruments, the sample and solvent use the same pipeline, which is easy to cause pollution. Especially for instruments without cleaning function, a lot of compounds will remain in the pipeline in a short time, which is the root cause of many experimental failures. . The automatic solid phase extraction instrument uses a flow separation unit to automatically select a solvent, and the organic solvent waste liquid has an independent waste liquid passage to effectively avoid cross contamination.
5. How do users choose the right solid phase extraction method?
The easiest way to establish a solid phase extraction method is to look it up from published standards or materials from SPE column manufacturers. However, since the sample matrix to be faced may vary and even the target compound is different, the methods provided in the literature are not necessarily the most optimized method. Therefore, the extraction parameters in these methods need to be optimized.
The establishment of a solid phase extraction method is divided into three steps:
(a) bringing together relevant information;
(b) establishing a preliminary solid phase extraction method;
(c) optimizing the parameters in the method;
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