Recently, many of our customers have reported encountering unscrupulous companies and small workshops that prioritize profit over quality. These entities are using counterfeit and substandard ELISA kits, which negatively impact the scientific community by undermining the integrity of research. They take advantage of the seriousness and rigor of scientific work while failing to uphold the same standards themselves. This not only disrupts research processes but also creates chaos in the market. As a responsible supplier, we feel it is our duty to expose these fake ELISA kits and provide reliable methods for researchers and users to identify them. We hope to bring attention to those who sell low-quality products, such as mislabeled imported reagents or completely fake items, so that the public can speak out against them.
Here are some effective methods to identify counterfeit ELISA kits:
1. Interference Test: This test helps determine if the ELISA kit detects the intended antigen. Follow these steps: (1) Prepare a mixed incubation solution with the kit’s recombinant protein antigen and detection antibody at a ratio of approximately 1:2. (2) Incubate the mixture at 37°C for 15 minutes. Instead of proceeding with the original kit's detection steps, continue with the following procedures. (3) Add the detection antibody, enzyme conjugate, and substrate according to the kit instructions. (4) After completing the experiment, analyze the standard curve. If the curve is clear and well-defined, it suggests that the added antigen does not match the kit’s antibody, indicating a counterfeit product. (5) If the standard curve appears distorted or non-existent, it may mean that the detection antibody was neutralized or interfered with by the antigen, suggesting the kit is genuine and capable of detecting the target antigen.
2. Cross-Experiment Test: If you have purchased two different ELISA kits (A and B) from the same company, you can perform a cross-experiment to check for authenticity. (1) Take two coated plates from kit A. (2) Use one plate with kit A to create a standard curve. (3) Use the second plate with kit B to create another standard curve. (4) Follow the kit instructions to add the detection antibody, enzyme complex, and substrate. (5) After the experiment, compare the two standard curves. If they are identical, it indicates that both kits are detecting the same parameter, which is unlikely for genuine kits, suggesting that one or both may be counterfeit. (6) If the curves differ significantly, it shows that each kit detects a unique target, confirming that they are legitimate ELISA kits.
By using these identification methods, researchers can protect themselves from being misled by substandard products. It is essential to remain vigilant and ensure the accuracy of the tools used in scientific research. We encourage everyone to stay informed and share this information to help maintain the integrity of the ELISA testing process.
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