**Notes on Human Glycocholic Acid (CG) Test Kit Instructions:**
1. Before starting the test, remove the kit from the refrigerator and let it reach room temperature for 15–30 minutes. This ensures optimal performance of the reagents. If the enzyme label is accidentally opened, place the strip in a sealed bag to maintain its integrity.
2. The concentrated washing solution may crystallize during storage. When preparing the diluted solution, gently heat it in a water bath until fully dissolved. This process does not affect the accuracy of the results.
3. Use a dedicated pipette for each step to ensure precision. Regularly check and recalibrate the pipette to avoid measurement errors. It’s recommended to complete sample loading within 5 minutes. For large numbers of samples, using an automated pipetting device is advised to improve efficiency and consistency.
4. Always prepare a standard curve during each test. It is best to run duplicate wells for better accuracy. If the sample OD value exceeds that of the first standard well, dilute the sample with a suitable factor (n times) before testing. The total dilution factor should be calculated as ×n×5 to ensure accurate quantification.
5. The sealing film is for single-use only. Reusing it may lead to cross-contamination between samples, which could compromise the reliability of your results.
6. Keep the substrate solution away from direct light. Exposure to light can degrade the reagent and affect the final readings.
7. Follow the manufacturer's instructions carefully. The final test results must be confirmed using a microplate reader, not visual estimation or other methods.
8. Treat all samples, washes, and waste materials as potentially infectious. Dispose of them according to local biohazard regulations to ensure safety.
9. Do not mix components from different batches of the reagent kit. Each batch is calibrated separately, and mixing may lead to inconsistent or unreliable results.
10. In case of discrepancies between the English and other language manuals, the English version takes precedence. Always refer to the official English manual for the most accurate and up-to-date information.
By following these guidelines, you can ensure the accuracy, reliability, and safety of your glycocholic acid testing procedure.
Pencil Case,Portable Pencil Case For Students,3 Layers Large Capacity Girl Pencil Case,Durable Pencil Pouch Large Capacity Pencil Case
Guangzhou Ecobag Packaging Limited , https://www.gdecobag.com