**Rat Factor VIII-Associated Antigen (FVIII-Ag) ELISA Kit – Instructions for Use**
**Kit Specification:**
Available in 48-well or 96-well configurations.
**Standard Diluent:**
1.5 ml × 1 bottle
**Enzyme Standard Reagent:**
3 ml × 1 bottle (for 48-well) / 6 ml × 1 bottle (for 96-well)
**Note:** This reagent is intended solely for research purposes. The standard curve is constructed by plotting the concentration of the standard on the x-axis and the OD value on the y-axis. Once the standard curve is plotted, the corresponding concentration can be determined based on the OD value of the sample. Multiply this value by the dilution factor to obtain the actual sample concentration. Alternatively, you can calculate the linear regression equation from the standard curve using the standard concentrations and their corresponding OD values. Then, substitute the OD value of the sample into the equation to calculate the sample concentration, and finally multiply by the dilution factor to get the true concentration.
**Kit Components:**
- Sealing Film: 2 pieces (for 48-well) / 2 pieces (for 96-well)
- Standard: 1 vial at 2700 ng/mL, 0.5 mL × 1 bottle
- Sample Diluent: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Developer A: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Chromogen B Solution: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well), store at -20°C
- Stop Solution: 3 mL × 1 bottle (for 48-well) / 6 mL × 1 bottle (for 96-well)
- Concentrated Wash Solution: 20 mL × 20 times (for 48-well) / 20 mL × 30 times (for 96-well), store at 2–8°C
**Storage Conditions:**
All reagents should be stored at 2–8°C unless otherwise specified. The chromogen B solution must be stored at -20°C.
**Preparation and Usage:**
Before starting the experiment, ensure all reagents are brought to room temperature. Follow the step-by-step instructions provided in the manual for accurate results. Always use clean pipette tips and avoid cross-contamination between samples.
This ELISA kit is designed for the quantitative detection of Rat Factor VIII-Associated Antigen in biological samples. It provides a reliable and sensitive method for measuring FVIII-Ag levels in research settings. Make sure to read the entire instruction manual before beginning the assay.
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