Shanghai Xinfansheng provides human basic fibroblast growth factor (bFGF) ELISA kit, which can provide free testing services, provide experimental reports for one week, and provide raw data analysis data. Welcome to contact.
Shanghai Xinfan Bio-Agent sells "human basic fibroblast growth factor (bFGF) ELISA kit" company with good market reputation, professional sales and technical service team, authoritative enzyme-free agency, professional technicians . "Human basic fibroblast growth factor (bFGF) ELISA kit" spot direct free home delivery, welcome to contact.
Shanghai Xinfan Bio provides ELISA kits for testing services. You can purchase the company's kits for free. The authoritative enzyme-free laboratory, senior experimental experts test, report and data for a week, to ensure the authenticity and reliability of the data, so that your experiment saves time and worry.
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There must be a complete plan before collecting samples, and it must be clear whether the ingredients to be tested are stable enough. Samples that were tested on the day after collection were stored in time at 4 °C for use. For samples that are retested every other day, they should be stored at -20 °C in a timely manner after sub-packaging. If conditions permit, it is best to freeze at -70 °C for later use. Specimens should avoid repeated freezing and thawing. Liquid specimens: including serum, plasma, urine, pleural and ascites, cerebrospinal fluid, cell culture supernatant, etc.
1. Serum: The blood is naturally coagulated at room temperature for 10-20 minutes and centrifuged for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if precipitation occurs during storage, it should be centrifuged again.
2. Plasma: EDTA, sodium citrate or heparin should be selected as anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again.
3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). The supernatant is carefully collected, and if a precipitate forms during storage, it should be centrifuged again. The chest and ascites and cerebrospinal fluid are implemented with reference to this.
4. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully.
5. Culture of cells: When measuring the components in the cells, the cell suspension was diluted with PBS (pH 7.2-7.4), and the cell concentration reached about 1 million/ml. By repeatedly freezing and thawing or adding tissue protein extraction reagents, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
6. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. Add a certain amount of PBS (pH 7.4), or tissue protein extraction reagent, and homogenize the specimen by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use.
7. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If you can’t get right now
For the test, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.
8. Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.
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