The BiomicV3 microbial identification instrument is produced by the United States Giles scientific company, using digital imaging technology for microbial inspection of special equipment. The device can read the three most common identification cards on the market: API (bioMerieux), Crystal (BD) and RapID (Remel) for microbial identification.
In order to ensure the accuracy of the inspection results, it is necessary to carry out effective quality control on the identification results during the installation and commissioning phase. The following uses the Meriere identification card API 20E as an example to illustrate the quality control process of Biomic V3. We know that conditions such as culture media, identification cards, reagents, handling and environment all have an impact on the accuracy of identification results. When it is determined that the above factors are suitable, the standard strains can be used to perform quality control tests to debug the software, so that the identification results are accurate.
The standard strains we used this time included Escherichia coli, Salmonella, and Enterobacter sakazakii. These three strains are very typical of three pathogenic bacteria in food quality and safety control; identification card API 20E is a pathogenic bacteria in food intestines. Identification card widely used in testing. The identification card is composed of 20 small tubes containing dry substrate. These measuring tubes are inoculated with a bacterial suspension. After culturing for a certain period of time, the color changes through metabolism, or the color changes after adding reagents to observe the results.
The test procedure is as follows:
(1) After cultivating the standard strains, single colonies are picked, added to NaCl 0.85% suspension and mixed into a bacterial suspension for later use.
(2) Fill the bacterial suspension with citrate CIT and VP GEL tubes with a pipette; other tubes only fill the tube part (not the cup part).
(3) ADH, LDC, URE, ODC and H2S are covered with mineral oil.
(4) Close the lid and incubate at 35 ~ 37 ° C for 18 ~ 24 hours.
(5) Add additional reagents to VP, TDA, IND.
(6) Put it in BIOMIC's drawer and interpret the result.
Test considerations include:
(1) Pick single colonies of pure culture. If there is an identification card that requires concentration, use a nephelometer to detect the required turbidity.
(2) Add a proper amount of water to the plastic shell containing the identification card to maintain humidity, avoid drying during cultivation
(3) It is best to use related reagents of Merrier, and do not use expired reagents.
(4) Pay attention when filling the sample, whether it is full of tube or cup.
(5) Before use, all additional reagents must reach room temperature (20-30 ° C).
(6) After use, all ampoules, test strips and culture boxes must be autoclaved, ashed or soaked in sterilant before disposal.
According to the image and data of the result, adjust the software, perform another quality control test according to the above method, and interpret the results. The final results are as follows:
Salmonella sp. 99.8% typical (ONPG-) (ADH +) (LDC +/-) (ODC +) (CIT +) (H2S +) (URE-) (TDA-) (IND-) ( VP-)
(GEL-) (GLU +) (MAN +) (INO +) (SOR +) (RHA +) (SAC-) (MEL-) (AMY-) (ARA +)
Enterobacter sakazakii Ent. Sakazakii The probability of results is 97.3% Very typical (ONPG +) (ADH +) (LDC-) (ODC +) (CIT +) (H2S-) (URE-) (TDA-) (IND-) ( VP-)
(GEL-) (GLU +) (MAN +) (INO +) (SOR-) (RHA +) (SAC +) (MEL +) (AMY +) (ARA +)
Escherichia coli E. coli has a probability of 99.6% results Very typical (ONPG +) (ADH-) (LDC +) (ODC +) (CIT-) (H2S-) (URE-) (TDA-) (IND +) ( VP-)
(GEL-) (GLU +) (MAN +) (INO-) (SOR +) (RHA +) (SAC +) (MEL +) (AMY-) (ARA +) .
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