Quantification of enzyme-labeled antibody and its molar ratio

Quantification and molar ratio of enzyme-labeled antibody When quantifying and calculating the molar ratio of enzyme-labeled antibody, the enzyme-labeled antibody should be purified as much as possible, otherwise it will affect the quantification of enzyme-labeled antibody and the calculation of molar ratio. The purification method generally does not use salting-out method, because the purity of the enzyme-labeled antibody prepared by the salting-out method is not high enough. If you want to prepare a high-purity enzyme-labeled antibody, it is best to use column chromatography. Quantification of enzyme-labeled antibodies. Spectrophotometry is generally used. Molar ratio of horseradish peroxidase-labeled antibody: if it is not HRP, but other enzymes, the molar ratio of enzyme-labeled antibody can also be obtained in turn when the molecular weight of the enzyme is known. The connection between enzyme and antibody is only a part of the connection between enzyme and protein.

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