Rare Sugar is a type of monosaccharide and its derivatives (the definition of ISRS in 2002) that exists in nature but has very little content. Its taste is similar to sucrose, but it has low calorie, high stability and sweetness. The advantages of coordination, no hygroscopicity, no caries, and high tolerance can make up for the shortcomings of traditional sweeteners and play an important role in improving the diet of special populations.
The typical representatives of rare sugar sweeteners are allulose and tagatose (the epimers of C-3 and C-4 of fructose, respectively). High and body fat accumulation, scavenging free radicals, neuroprotection, modifying drugs or active substances to optimize its functional activity, and other aspects play an important role in physiological activity. It is a new sweetener for people with diabetes and obesity. Because of the small amount in nature, it is difficult to obtain by purification, and chemical synthesis is expensive. Therefore, the use of biotransformation technology to develop functional scarce sugar has become a hotspot in international research.
The functional sugar and natural active substance research group led by researcher Sun Yuanxia of the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, after extensive screening and functional gene mining, discovered the conversion of D-tagatose and D-aloxone using cheap monosaccharides as substrates Two new key enzymes for the biotransformation of rare sugars with independent intellectual property rights of sugar, in-depth study of the kinetic properties of the enzyme, and the analysis of the crystal structure of its protein molecule, revealing the conversion of hexanone sugar epimerase The mechanism of action of scarce sugars has constructed engineering strains suitable for large-scale production to obtain recombinant isomerases with good stability. At the laboratory level, the conversion rates of the two sugar isomerases reached 30% and 60%, respectively.
The research on the discovery of key enzymes, enzymatic properties and conversion efficiency in the method of biotransformation of rare sugars will lay the foundation for the realization of large-scale biological conversion of rare sugars.
Related research results were published in academic journals Extremophiles and Protein & Cell.
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