Human Endothelial Mononuclear Cell Activated Peptide II (EMAP-II) Technical Method

[Human endothelial mononuclear cell activated polypeptide II (EMAP-II) technology method] LBS agar rat basic fibroblast growth factor 9 (bFGF-9) production elisa kit rat protein phosphatase (PP) production elisa kit Rat angiopoietin 2 (ANG-2) production elisa kit modified Camp-BAP agar base mouse neutrophil elastase (NE) production elisa kit rat Toll-like receptor 4 (TLR4) production elisa kit Human dopamine-beta hydroxylase (DBH) production elisa kit [Human endothelial mononuclear cell activated polypeptide II (EMAP-II) technique] Determination of endothelial mononuclear cell activated polypeptide II (EMAP) in human serum, plasma and related liquid samples -II) content. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human endothelial mononuclear cell activated polypeptide II (EMAP-II) in the specimen. The purified human endothelial mononuclear cell activated polypeptide II (EMAP-II) antibody was coated with a microplate to prepare a solid phase antibody, and the endothelial mononuclear cell activated polypeptide II (EMAP-II) was sequentially added to the microcapsule of the coated monoclonal antibody. Then, it binds to HRP-labeled endothelial mononuclear cell activated polypeptide II (EMAP-II) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with substrate TMB to develop color. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with endothelial mononuclear cell activated polypeptide II (EMAP-II) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the content of human endothelial mononuclear cell activated polypeptide II (EMAP-II) in the sample was calculated by a standard curve. [Human endothelial mononuclear cell activated polypeptide II (EMAP-II) technology method]

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